It's important to know about these effects, and they have been measured. See this link about the Fletcher-Munson curve:
The Fletcher Munson Curve & Why You Should Know About It |ZeSoundSuite Dot Com
Human ears do not best hear all the frequency range at the same loudness. There's a reason why around 85dB SPL (sound pressure level) is recommended. (A Rolling Stones concert would probably be over 100dB in contrast).
So here's the skinny on what I recommend - get an SPL (sound pressure level) meter. One can be had from Radio Shack for under $100. Might be something cheaper out there nowadays too.
Setup both monitor outputs (playing same time) to peak around 80-85dB on the SPL meter at your listening position That's your fallback volume reference. I recommend getting something that gives you a manual volume control knob so you can mark that setting on the knob (i.e. TC Level Pilot, Mackie Big Knob, etc.).
When you're just listening to a track for initial problems, like noise, clicks, pops to do edit cleaning, I recommend use of headphones for that stage (if you have a good set of cans). You can hear those things better under cans. Might save your monitors too when doing EQ boost sweeps to find those things. When each track is done, take the cans off and use your monitors.
When you're mixing, don't just pull all the faders up and leave them there for hours, never stopping the audio. Make your adjustments 'offline' (without the mix playing). Then play and see what changes it made, then stop it again, guess where it needs to be effected, make the adjustment, then play it again for a few seconds, then stop it again, tweak some more, play, stop, tweak, etc. Get into a tweak, play, stop, tweak rhythm, because that will keep your ears from getting used to any one loudness so you can hear what the tweak did. And it will also sharpen your skills to hear in your mind what tweaking a track needs, and eventually, you'll be able to listen to a track for just a few seconds and automatically hear what or where it needs addressing.
With the mastering stage, I know many engineers match the levels between a pre-adust and post-adjust tweak. They pay strict attention to keeping the loudness output the same as it was before they applied the treatment. Doing that makes sure you can hear exactly what the tweak did if anything. I believe WaveLab has a tool just for doing this.